Recent advances in solid phase microextraction with various geometries in environmental analysis

Solid phase microextraction (SPME) has emerged as a versatile sample preparation technique for the preconcentration of a broad range of compounds with various polarities, especially in environmental studies. SPME has demonstrated its eco-friendly credentials, significantly reducing the reliance on solvents. The use of biocompatible materials as a coating recipe facilitates the acceptance of SPME devices in analytical chemistry, primarily in the monitoring of environmental pollutants such as persistent organic pollutants (POPs), volatile organic compounds (VOCs), and pesticides from the various environmental matrices. During the last few years, investigators have reported an improvement in the SPME enrichment technique after changing the coating recipe, geometries, and sampling procedure from the complex matrices. Furthermore, the development of various geometries of SPME with large surface areas has enhanced the extraction efficiency of environmental pollutants. As a miniaturized sample preparation technique, SPME significantly reduces the solvent usage, suggesting a potential platform for green chemistry-based research for water, air, and soil analysis. This review article summarizes the evolution of SPME, its various modes, the application of SPME, recent innovations, and prospects for the determination of water, air, and soil pollution. The advantages and disadvantages of SPME in comparison to other extraction techniques have been discussed here. This review serves as a valuable resource for investigators working in sustainable environmental research.


Introduction
The year 1987 marked the introduction of the concept associated with sustainable eco-development by the World Commission on Environment and Development.Therefore, it is important to preserve the natural resources for the current and future generations.A call was made for balanced development, urging that economic progress must not come at the expense of environmental protection and public health. 1Thus, the sustainable approach is particularly relevant for chemists, who can implement "green chemistry" principles within the laboratory and industrial settings for the optimal utilization of resources.As a result, "Green Analytical Chemistry," or GAC, emerged in 1999 and has gained popularity, quickly becoming a commonly used term in the chemical sciences.Several research papers have been published in the same area, highlighting the importance of this theory.GAC highlights the usage of environmentally friendly sample preparation techniques at every stage of the analytical method. 2 It also emphasizes the "3Rs" principle.This principle involves replacing the harmful solvents with greener alternatives, reducing the number and quantity of solvents used, and recycling solvents wherever possible.By following these principles, GAC minimizes the harmful impact on the environment due to the use of less solvents during the sample preparation and facilitates the widespread research using natural resources. 3enerally, an analytical process involves ve steps: selection of sample matrix, sample preparation, separation, detection, and analysis of acquired data.These analytical processes serve as a valuable tool for ensuring stability and quality for treating the analytes. 4Sample preparation is considered as a crucial process since it consumes 80% of the time duration in the entire sampling process and nally inuences the research output during the analysis.
Initially, the solid phase extraction (SPE) technique was derived as a powerful technique for the extraction of trace quantities of analytes present in the sample matrix.Also, SPE minimizes the use of solvents compared to the traditional liquid-liquid extraction (LLE), which still requires organic solvents and poses a concerning safety issue. 5Moreover, optimization of SPE for targeting the specic analytes and matrices can be challenging and time-consuming. 6Therefore, an alternative sample preparation technique other than SPE is required to minimize the risks associated with the traditional solvents, and the fast analysis of the samples. 7Accordingly, the landscape of analytical chemistry has recently been enriched by the emergence and successful application of miniaturized extraction procedures by techniques such as liquid-phase microextraction (LPME) and solid-phase microextraction (SPME).These methods can leverage the exceptional capabilities of microscopic liquid or solid phases to effectively capture and concentrate analytes of interest. 8,9n ideal sample preparation method should be easy to operate, economically feasible, less time-consuming, environmentally friendly, and reproducible without losing its analytes.SPME is a technique used for sample preparation and extraction of analytes from the various matrices using the principle of absorption/adsorption through the solid/liquid phases.The extraction of analytes takes place according to the equilibrium partition theory, and the subsequent desorption of the concentrated analytes in chromatographic or other analytical devices allows for the detection of those compounds.Generally, SPME has two types of conformation: static in-vessel, where the extraction phase coating is present on the outer surface, and dynamic in-ow, where the sample is made to ow inside a capillary containing the stationary extraction phase. 10he SPME technique requires a small number of samples during the sample preparation process, suggesting less impact on the original sample matrix than the conventional SPE technique.Researchers focused on the strategies such as safe and non-toxic extraction to tackle the challenges associated with the sample preparation.Thus, this microextraction technique combines sampling, extraction, and enrichment into a single, streamlined process.These approaches effectively extract the target compounds from complex sample matrices for efficient and eco-friendly analysis at trace levels. 11ue to its eco-friendly nature, SPME unsurprisingly became a popular choice for environmental analysis.Early applications focused on detecting volatile organic compounds (VOCs), persistent organic pollutants (POPs), and pesticides in water, primarily using polydimethylsiloxane (PDMS)-coated bers.
Although PDMS acts as a good adsorption material, it has a lack of selectivity for specic analytes.SPME coated with sorbent materials, including carbon nanotubes, metal-organic frameworks and molecularly imprinted polymers, offer high selectivity and act as a promising platform for water, soil and sediments, and air sample analysis. 12These advancements solidied SPME as a transformative tool for environmental research, promising to provide a deeper insights and more efficient method. 13his review article presents an overview of recent advances in SPME and its applications in detecting environmental pollutants.The article outlines the latest developments in the SPME technique that make it suitable for environmental analysis of air, water, soil, and sediments.It also discusses the strengths and limitations of the technique.Additionally, this article provides a holistic view of SPME, considering its geometries, conguration, analytical techniques and the analytes detected in various aspects of environmental applications.Researchers will nd this comprehensive review highly informative as it delves deeper into the crucial role of SPME in modern analytical chemistry.

Chiranjit Ghosh
Chiranjit Ghosh is an Assistant Professor at the Manipal Institute of Technology, part of the Manipal Academy of Higher Education (formerly Manipal University).He is an analytical chemist, and his research area is sample preparation, mass spectrometry, material chemistry, clinical chemistry, metabolomics, biomedical science, and instrumentation.At present, Dr Ghosh's team is developing chemical microsorbent devices, called thin lm membrane and needle trap devices, using solid phase microextraction techniques for capturing metabolic marker compounds associated with various diseases.

Solid phase microextraction techniques
Microextraction techniques represent an advanced approach in sample preparation of analytical chemistry. 14The ber coating in SPME device acts as a selective sorbent for capturing analytes from the sample matrix. 15During the preconcentration stage, SPME ber is exposed into sample matrix to enrich with polar and non-polar compounds depending on the coating materials of the ber.Later, the ber is dissolved into the analyzer for the quantication of the analytes. 16,17Fig. 1 demonstrates the SPME coating procedure using covalent organic frameworks.
SPME offers two extraction approaches: equilibrium and preequilibrium stages.Equilibrium is a relatively slow process with high precision, whereas pre-equilibrium is comparatively faster and captures more volatile analytes.The selection of preequilibrium and equilibrium depends on the various inuencing factors, including the volatility of analytes and sample matrix complexity. 18rom the fundamental science point of view, SPME operates through the diffusion of the compounds from the sample matrices to coated materials as per the following equation: 19 n eq e ¼ where n eq e = amount of analytes extracted by the extractant.V e = volume of the extractant.V s = volume of the sample.C 0 s = initial concentration of the analyte before it reaches the equilibrium.
K es = partition coefficient or distribution coefficient.

SPME coating
The selection of SPME coating is important for acquiring effective extractions from different sample matrices.To select the appropriate coating materials, various properties of analytes, including polarity and volatility, analyte size and composition, should be considered.The widely used coating materials for traditional SPME applications are polydimethylsiloxane/ divinylbenzene (PDMS/DVB), carboxen/polydimethylsiloxane (Car/PDMS), polydimethylsiloxane/carboxen/divinylbenzene DVB/CAR/PDMS, polyacrylate (PA), and CARBOWAX polyethene glycol (PEG). 20However, the applications of SPME bres are sometimes limited due to the swelling effect of the bers by the solvents.Therefore, the researchers further explored the alternative coating recipes to enhance the mass transfer process and enrichment factors during the analysis.Recent advances in material chemistry have facilitated the development of a wide range of sorbent coating materials for various applications.During the last few years, several coating materials, including carbon nanotubes (CNTs), graphitic carbon nitride and boron nitride nanotubes/sheets, metal-organic frameworks (MOFs), zeolitic imidazole frameworks (ZOF), covalent organic frameworks (COFs), aptamers, molecularly imprinted polymers (MOF), ionic liquids (IL), and biocompatible SPME coatings have been utilized 21 for wide variety of applications.CNT coating is used to extract hydrophobic compounds.However, researchers utilized the functionalized CNT for extraction of polar compounds to capture pesticides, polyaromatic hydrocarbons (PAH), pharmaceuticals, etc. 22,23 The successful application of SPME with CNT with polypyrrole/titanium oxide coating materials was the extraction of analytes from the water used. 24The coating materials are usually immobilized on the solid substrates through methods such as sol-gel chemistry, dip coating, electrolytic coating etc.In order to perform rm binding of coating materials on a substrate, researchers exploited suitable binders like polyacrylonitrile. 24To achieve effective performance using the SPME technique, it is important to optimize the coating thickness and percentage of sorbent materials within the coating recipe.Thus, the thickness of the coating may inuence the time required to attain equilibrium and the desorption process during sample preparation.

Sampling modes of SPME
Generally, SPME offers three distinct sampling modes: direct immersion, headspace extraction, and membrane-protected extraction.Each mode caters to target the specic analyte characteristics and sample matrices during the sample extraction.Fiber SPME (Fig. 3A) presents three distinct extraction modes (DI-SPME, HS-SPME, and m-SPME) whose efficiency is intricately linked to certain experimental parameters like agitation, salt effect, and pH. 25 2.2.1 Direct immersion solid phase micro-extraction (DI-SPME).In the direct extraction mode of SPME (Fig. 2a), the coated ber directly interacts with the sample matrix to target the analytes.This interaction enables the rapid analyte transfer from the sample to the extraction phase. 26However, efficient extraction depends on the diffusion limitations, particularly in the case of liquid samples, where a stagnant layer of solution can form around the ber and it can hinder the movement of analytes.In aqueous samples, stirring or sonication disrupts the dense liquid layer surrounding the ber, known as the "depletion zone," and it may enhance the mass transport of analytes toward the ber surface.Contrarily, in case of gaseous samples with inherent air ow (e.g., convection), equilibration of volatile analytes might occur aer certain time without the need for the agitation due to the constant movement of the air molecules. 27Moreover, the DI-SPME offers several advantages, including simplicity, cost savings, and adaptability to different sample matrices such as solid, liquid, and gas. 28.2.2 Headspace solid phase micro-extraction (HS-SPME).Headspace extraction (Fig. 2b) is a versatile technique that can extract volatile analytes from the equilibrated vapour phase above the sample, thereby avoiding direct contact with the sample matrix.29 This adaptability allows the method to safeguard the ber coating from any physical damage, even while dealing with complex matrices like non-volatile, high molecular-weight constituents like proteins.30 Headspace SPME has several practical applications in analyzing diverse organic compounds across matrices such as biological samples, water, soil, and air.Its unique strength lies in the minimal extraction volume, which enables the efficient concentration of trace compounds onto the ber coating.31 However, certain organic compounds pose a challenge due to their strong affinity for the sample matrix.Researchers successfully overcame this hurdle aer the involvement of various coating materials for strong interaction between the compounds of interest and the coating substances.The practicality of SPME is evident in its integrated design, which seamlessly transfers the absorbed compounds directly into the GC injector aer the extraction and concentration.The selective absorption properties of the ber coating act as a barrier, preventing oxygen and moisture from interfering with the GC column.This feature provides a critical advantage over direct SPME, particularly for analyzing complex matrices like greasy/ oily water and human blood. 32A study by Rosalia et al. used the HS-SPME method to determine volatile compounds from mozzarella cheese following the x-ray irradiation.33 They observed the maximum efficiency of extraction of the volatile compounds by DVB/CAR/PDMS SPME ber.Here, the volatolomic changes from the sample matrix may be the indicators to verify the quality of mozzarella.The study was reported to be an important technique for monitoring the quality of cheese samples. 34o check the analytical performance of HS-SPME ber for the presence of untargeted metabolites in the urine matrix, researchers utilized DVB/CAR/PDMS, DVB/PDMS, CAR/ PDMS, PA, and PEG bers.They reported the DVB/CAR/PDMS as a better recipe for the extraction of compounds for the mentioned purpose.The study also reported the presence of 4methylphenol, 4-heptanone, phenol, dimethyl disulphide, and dimethyl trisulphide compound in the urine matrix.35 2.2.3 Membrane protection solid phase micro-extraction (m-SPME).Membrane-protected solid-phase microextraction (m-SPME) is a widely used analytical technique that has successfully incorporated a diverse range of solid sorbents for capturing the analytes from both the liquid and solid matrices.This adaptability is evident in the use of various materials, including carbon nanotubes, 36 commercially available C 18 bonded silica, 37 graphite ber, and activated carbon.Unlike the conventional SPME for complete analyte extraction, m-SPE operates at an equilibrium, which limits the maximum extractable amount.38 In comparison with other techniques, membrane-protected SPME (Fig. 2c) stands out by introducing a selective membrane between the ber and the sample, effectively blocking the interferences.This unique feature is particularly benecial when dealing with highly contaminated samples, as the protective barrier shields the ber from the destructive effects of high-molecular-weight compounds. Thgh the headspace extraction offers similar protection, membrane protection extends its reach to less volatile analytes, thereby broadening its analytical scope.However, the extraction process becomes signicantly slower than the direct immersion due to the additional diffusion hurdles imposed by the membrane.Fortunately, this drawback can be mitigated by employing the thin membranes and increasing the extraction temperature, which leads to a shorter analysis time.39 The signicant sensitivity differences between the direct and headspace sampling are primarily observed in the case of highly volatile analytes.In situations where sensitivity limitations arise due to the low analyte concentration, additional steps like dispersive liquid-liquid microextraction (DLLME) can be introduced.40 SPME remains the most prevalent due to its simplicity of use, versatility, and compatibility with diverse analytical platforms including the gas chromatography (GC), liquid chromatography (LC) or other hypenated techniques.40,41 3. SPME configurations 3.1 SPME ber SPME offers a plethora of commercially available coatings and ber congurations, each designed to cater to diverse analyte types and sample matrices.Depending on the convenience, the SPME encompasses various congurations: ber, stirrer, vessel wall, suspended particle, tube, and membrane.The emerging techniques like cold SPME ber, which utilizes a ber that are preconditioned at a lower temperature, whereas hot-water SPME, uses high temperatures to attain an improved level of desorption, and broaden the optimization landscape for the analytes.42 The popular coating materials of the SPME ber including PDMS provides for widespread applicability for polyacrylate (PA), divinylbenzene (DVB) 43 for enhanced thermal stability, carboxen (CAR) 44 for strong affinity towards aromatic compounds, and carbowax (CW) for capturing polar analytes.These coatings can be applied in varying thicknesses and formats, including single coatings, mixtures, and co-polymers for improving the selectivity and extraction efficiency.The diverse coating materials empower the SPME ber applications across a broad spectrum of analytes, encompassing non-polar organic compounds (e.g., BTEX, PAHs, pesticides) 45,46 and polar organic compounds (e.g., phenols, alcohols, ketones, nitroaromatics).Among the industries, Supelco (Bellefonte, PA, USA) that has pioneered SPME metal ber assemblies featuring a metal alloy core for enhanced ber lifetime, durability, and extraction reproducibility of the metabolic analytes.47 Importantly, choosing the optimal coating for a specic application is not just a step but a crucial decision in maximizing the efficacy and versatility of SPME.However, due to the limited availability of commercially available stationary phases can restrict the broad range applicability of the SPME ber technique.Wu et al. developed a polypyrrole (PPY) coating, specically tailored for extracting the polar and ionic groups of analytes.42 This research emphasized that the stationary phase of the ber must exhibit high affinities for the polar analytes and should possess exceptional stability in the diverse solvent environment.48 An advancement in sol-gel technology has helped to revolutionize the SPME ber design by offering a robust platform for effortless integration of organic functionalities within the inorganic, polymer-based structures within the solution phase.49 The reported bers exhibited an exceptional resilience even in the harsh environments, resisting the degradation in both strong organic solvents and acidic solutions.50 This process involves a chemical reaction that transforms a liquid (sol) into a solid (gel) network, allowing for the development of a customized coatings.This breakthrough paves the way for the generation of diverse coatings to analyze environmental pollutants, as shown in Table 1.

In-tube SPME
In-tube SPME utilizes a segment of the capillary column as an extraction device. 58In-tube SPME (Fig. 3B) offers a miniaturized extraction capillary column.In accordance with the partition equilibrium, the analytes from the dilute solution are preconcentrated into the stationary stage by repetitive draw/eject phases. 59The extracted analytes can then be immediately transferred into the liquid/gas chromatography column. 60Intube SPME has recently been used with miniaturized LC techniques to reduce the solvent usage while improving the column efficiency by providing a better compatibility.It has been utilized to assess different kinds of environmental samples.For instance, a study by Mora et al. 61 developed a new method, IT-SPME-nanoLC, which is suitable for the breakdown of herbicide tribenuron-methyl (TBM) in various water sources.They observed that TBM remains stable in ambient water for weeks.However, the degradation rates vary depending on the source.In the future, the optimisation of in-tube SPME lies in developing different types of capillaries for capturing and isolating target analytes.Researchers are actively experimenting with various capillary designs like wall-coated, 62 porous layer, 63 sorbet packed, 64 monolithic rod 65 and ber-packed capillaries. 66

Recent applications of SPME in environmental analysis
SPME is an efficient tool for analyzing environmental pollutants across diverse matrices, including air, water, soil, and sediment, both on-site and off-site.Its popularity is evident in the numerous recent publications that have successfully applied the SPME to their environmental analysis.Tables 2-4 provides information about the wide range of applications of SPME for gaseous, aqueous, and solid matrices, respectively.

Air samples
The monitoring of air for the presence of trace contaminants currently relies on techniques that are inhibited by inherent drawbacks compromising their usefulness and accuracy. 121The approaches such as grab or spot sampling in canisters or nylon bags followed by direct concentration over a sorbent bed using portable pumps and passive diffusion monitors is a potential method, but they are primarily limited due to their high operational time and expensive cost. 121Additionally, these methods heavily rely on sorbent materials like charcoal, silica, and polymers, whose breakthrough volumes have demonstrated a signicant susceptibility in response to humidity uctuations.Furthermore, the traditional desorption step, typically employing solvents, adds further complexity to the workow and that can introduce contamination risks during the sample preparation.SPME presents an effective possibility for signicantly enhancing the cost-effectiveness of air analysis by consolidating the initial analytical steps: sampling, extraction/concentration, and convenient delivery to the instrument.This could lead to substantial cost savings in environmental monitoring. 122Table 2 summarizes the comprehended collection of recent SPME-based approaches for analyzing the airborne environmental contaminants.
A study by Weini et al. utilized an SPME technique coupled with portable GC-MS for monitoring the air pollutants. 123The study had several advantages compared to the use of benchtop GC-MS, including the miniaturization and determination of air pollution in remote locations where it is challenging to reach physically for sample collection.Researchers utilized MOF-801 as an active material for fabricating SPME ber and utilized it for the estimation of the BTEX (benzene, toluene, ethylbenzene, xylene) contents from indoor air samples.Interestingly, the investigators claimed the customized SPME ber production cost might be around $20, which is signicantly more costeffective than the commercial counterparts. 124A previous study by Anara Omarova et al. reported the feasibility of MOF-  199-based SPME ber for the monitoring of BTEX compounds from the air. 125The technique showed high reproducibility and repeatability with a limit of detection around 0.03 mg m −3 .SPE-SPME-GC/MS/MS measured PAHs 126 and antioxidants to check the indoor air quality. 127The combined technique of SPE and SPME was able to extract PAH contents up to 51 ng m −3 .Apart from this, the authors reported their success for the estimation of benzothiazole (BTZ), diisobutyl-dibutyl-and di-(2-ethylhexyl)phthalate at a low concentration range, suggesting a fast and sensitive method for extraction of multiclass compounds with various chemical moieties for indoor and outdoor air quality check. 128A 'gas-cycle-assisted system' was developed with a mini-pump, glass vial, silicone tube and speed controller and SPME ber for quantication of organic pollutants including PAHs, polychlorinated biphenyls (PCBs), and ve phthalate esters (PAEs).The proposed technique increased the extraction efficiency of semi-volatile compounds for the HS-SPME analysis. 129The proposed method was successful in the determination of air pollutants, including benzene, 5methylhexanophenone, acetophenone, para-alphadimethylstyrene, pinane, beta-myrcene, menthol and derivatives, limonene, eucalyptol, meta-divinylbenzene, and paraethylstyrene.Esther Borrás et al. reported a SPME derivatization technique for monitoring of various compounds, including aldehyde, ketones, hydroxy-aldehyde and carboxylic acid. 130The study demonstrated excellent efficiency with a limit of detection within the 6-100 pptv range.The main advantage of the study is that it was able to monitor the real-time trace levels of multioxygenated compounds in a short time.To enhance extraction efficiency, researchers utilized SPME ber with a metal-organic frameworks (MOF) and nanoparticles-based coating for the environmental research.

Water samples
The application of SPME for analysis of water pollutants have been widely discussed.The ber-retracted device, initially designed for time-weighted average air sampling, has been cleverly adapted for water.Replacing the air present in the needle with water and employing a gas-tight syringe ensures the sample integrity and minimizes the analyte carryover.These approaches demonstrate the versatility of SPME and its potential for continuous water monitoring, opening the doors for comprehensive environmental analysis. 131ollow ber SPME (HF-SPME) with commercially available and laboratory-coated capillary columns have been used to analyze triazines and arsenic in aqueous samples [37-42].Techniques like GC-MS, GC-FID, and AFM have been used to analyze PCBs, PAHs, and arsenic, respectively, as well as other compounds in aqueous samples.In addition to the commercial SPME devices, bers with new coating materials, including OTMS (octadecyl trichlorosilane), 80 ZIF-8/h-BN (zeolitic imidazolate framework-8/hexagonal boron nitride), 82 mixed C18/SCXcoated, 95 have been used for analysis of environmental analytes in water samples.Beyond the traditional coating of bers, researchers explored novel coating materials to enhance the selectivity and sensitivity of the SPME device.For instance, a study by Tan Lei Lu et al. performed a study where they utilized the pipette tip SPME technique for the monitoring of water pollutants, including sulfachlorpyridazine, sulfamethoxazole, and sulfadimethoxine from water samples. 132The study used the activated charcoal as an active material to fabricate the microextraction tool.It demonstrated the limit of detection around 1 ppb and good linearity of the calibration curve in the concentration range of 5-500 mg L −1 .The advantage of the study is that it eliminates the sorbent synthesis and requires a low chemical consumption for the extraction process.Another recent study utilized chitosan cross-linked graphene oxide aerogel for coating SPME ber and they successfully utilized it for the extraction of PAH.This study reported a low LOD value of 0.5-1000 ng L −1 of PAH from water matrix. 133Researchers utilized the direct immersion technique by polymeric ionic liquid sorbent coating of SPME bers for the estimation of water pollutants.Karla Vargas-Berrones et al. proposed a lowcost technique for quantication of 4-nonylphenol in water. 134hey utilized N-methyl-bis(triuoroacetamide) (MBTFA) as a derivatizing agent on SPME ber that can be coupled with the GC-MS for monitoring of 4-nonylphenol, an endocrine disruptor compound present in water.The study was useful for the cost-effective estimation of 4-nonylphenol.A carbon nanomaterials-based SPME device was developed for the extraction of 24 pesticides, including triuralin, phorate, atrazine, propazine, diazinone, and napropamide. 135Researchers reported the presence of the 4-4-n-pentylphenol, 4-n-hexylphenol, p-tert-octylphenol, and nonylphenols in river waters by utilization of SPME ber. 136The study exploited the stainless steel/ionic liquid SPME to monitor alkylphenols.Table 3 gives a summarized version of the analysis application of SPME in aqueous samples.

Soil and sediment samples
When collecting soil and sediment samples with SPME, researchers directly immerse the ber (DI) or extract volatile compounds from the vapour emitted by the sample (HS).They oen use additional techniques like sonication (sound waves), microwaves, or heating/cooling to increase extraction efficiency.Most studies rely on traditional calibration methods to quantify the extracted compounds.However, some HS-SPME analyzes of BTEX 112 in soil utilized a specic calibration technique, which is  137 To enhance the efficiency of SPME-based extraction, researchers utilized the carbon nanospheres coated ber for estimation of PAHs including naphthalene, acenaphthene, phenanthrene from soil and water matrices.The method reported the recoveries of PAH hydrocarbons from the water around 80-12% with a standard deviation of less than 13.9%. 138urther, Dina Orazbayeva et al. reported the feasibility of headspace SPME for monitoring epoxiconazole, metribuzin, uroxypyr, and oxyuorfen pesticides from soil.The study showed a promising way for determining pesticides from a complex matrix like soil. 139The PAHs were measured with the SPME technique with good linearity (40-4000 ng g −1 ) with low LOQ from 4.2 to 8.5 ng g −1 .Ferrocene and ve derivative compounds (ferroceneacetonitrile, ferrocenecarboxaldehyde, and benzoylferrocene, 1,1 0 -dimethylferrocene, and acetylferrocene) were measured by direct immersion of DVB/CAR/PDMS coated SPME ber coupled to atomic emission detection spectrometry.The study did not nd any matrix effect during the seawater sampling by SPME. 140However, due to the complexity of the medium, the standard addition method should be considered for the soil samples.
Further study by Xinze Wu et al. investigated the extraction efficiency by Zn5 cluster-coated SPME bers for extracting ten phenolic compounds in soil samples.The work reported good linearity and low LOD using metal coordination cluster coating on SPME bers. 141Qian Yan utilized the covalent organic framework-derived carbon as a coating recipe for SPME for the estimation of PAHs from soil, highlighting the applicability of this material as an SPME coating recipe for the determination of PAHs from the soil matrix 142 which can open up a new avenue for SPME research.Table 4 explains the various applications of SPME in analyzing soil and sediment samples.

SPME for monitoring of inorganic pollutants
The detection and quantication of inorganic pollutants are critical in environmental research to ensure and monitor the quality of water, soil, and air samples.Inorganic pollutants such as heavy metals threaten human health.However, the traditional method of detecting these pollutants uses large amounts of hazardous solvents, making the process labour-intensive and cause environmental degradation.To overcome these limitations, SPME has emerged as a powerful green alternative for extracting and preconcentrating the inorganic pollutants from various matrices present in sparse quantities with high sensitivity and precision coupling with other analytical instruments. 143 a result of recent advancements, environmental monitoring using SPME has gained immense importance.The nanoclay demonstrated the outstanding efficiency in adsorbing and eluting Cu(II), Cd(II), and Pb(II) at pH 2, using nitric acid for elution. 144The microextraction method exhibited high recovery percentages across a range of sample volumes from 15 mL to 70 mL.The limit of detection and limit of quantication values for Cu(II), Cd(II), and Pb(II) were found to be around 1.8, 1.3, and 1.9 mg L −1 and 5.3, 3.9, and 5.7 mg L −1 , respectively.The recovery percentage from various drinking water samples consistently lies within the range of 88-105%.
Magnetic solid phase microextraction (m-SPME) utilising Fe 3 O 4 nanoparticles (NPs) was developed for the preconcentration and analysis of cobalt and copper in natural samples such as plants, aqueous and soil samples employing microsample injection system-ame atomic absorption spectrometry (MIS-FAAS).This approach achieved recoveries of around 95% Co(II) and 80% for Cu(II) from 90 mL of the sample.The extraction time was around 10 minutes, with the preconcentration factors of 180 for both Co(II) and Cu(II).The method yielded the low detection limits with values of 1.2 mg L −1 and 0.9 mg L−1 for Co(II) and Cu(II), respectively.The analysis was validated with the standard reference materials, including TMDW-500 drinking water, NIST 1573a tomato leaves, and NCS DC 78302 tibet soil and the method demonstrated recoveries >95% in spiking experiments. 145n addition, a miniaturized SPME system was designed as a simple and efficient method for concentrating cadmium (Cd) in the biological and environmental samples matrices, followed by the FAAS analysis.Then, the sample solution was treated with a complexing reagent that was drawn into the syringe and cycled through the packed tip.The analytes were extracted and sent for analysis.The technique exhibited good sensitivity and repeatability.It was successful in determining the cadmium levels available in drinking water and biological samples from the patients with the renal malfunction and healthy controls.The proposed approach was rapid and it had minimal contamination risks and was more cost-effective compared to the traditional methods.Additionally, it eases the sample preparation technique by eliminating the need for centrifugation, use of few samples and nally consumption of less solvents.This method offers a practical tool for screening and quantifying metal ions present in the various samples.The study also revealed the excess level of cadmium in the underground water and it can affect the renal function. 146n a further study, the researchers proposed a novel method for chromium speciation by integrating magnetism-assisted intube solid-phase microextraction (MA/IT-SPME) in combination with a high-performance liquid chromatography-diode array detector (HPLC-DAD). 147The method involved the coordination of chromium species Cr(III) and Cr(VI) with ammonium pyrrolidine dithiocarbamate (APD) to form the complexes.The method consisted of a microextraction column containing a porous monolith doped with magnetic nanoparticles constructed within a capillary, surrounded by a magnetic coil to generate variable magnetic elds during the extraction process.The study showed that the magnetic eld signicantly enhanced the extraction efficiency for Cr(III)/APD (80.4%) and Cr(VI)/APD (86.2%) complexes.The method achieved detection limits of 0.0059 mg L −1 for Cr(III) and 0.0020 mg L −1 for Cr(VI) in water samples and 0.47 mg kg −1 for Cr(III) and 0.057 mg kg −1 for Cr(VI) in soil samples.The technique effectively quantied Cr(III) and Cr(VI) at trace levels in the environmental water and soil samples, demonstrating its practical applicability for the chromium speciation.
Furthermore, investigators developed a cost-effective green analytical technique for the detection of tributyltin (TBT), which is considered one of the POPs. 148TBT is known to impact aquatic animals in Sri Lanka.The study utilized a HS/SPME-GC/ MS technique as a sensitive, simple, and solvent-free method.This technique allowed the researchers to quantify the TBT at very low levels in the various environmental samples.The study was able to quantify the TBT down to the 1 ppt level, which is below the contamination levels set by the WHO.The research work highlighted the practicality of SPME in detecting and quantifying TBT contamination in coastal environments.
The nano-clay demonstrated outstanding efficiency in adsorbing and eluting Cu(II), Cd(II), and Pb(II) at pH 2, using nitric acid for elution. 144The microextraction method exhibited a high recovery percentage across a range of sample volumes from 15 mL to 70 mL.The limit of detection and limit of quantication values for Cu(II), Cd(II), and Pb(II) were found to be around 1.8, 1.3, and 1.9 mg L −1 and 5.3, 3.9, and 5.7 mg L −1 , respectively.The recovery percentage from the regular water samples consistently lies within the range of 88-105%.
In further research, a self-heating HS-SPME device was proposed using a gold-coated tungsten ber for the detection of mercury in soil through the miniature point discharge optical emission spectrometry (mPD-OES).This method reduced the desorption time and power consumption signicantly compared to the traditional external heating methods.The Au@W SPME ber effectively preserved the mercury for longterm storage, with a sample loss of less than 5% over a onemonth period.The accuracy of the device was validated using a soil-certied reference material and nine soil samples with the reported recovery rates ranging from 86% to 111%. 149In addition, the magnetic mesoporous carbon (Fe 3 O 4 @C, MMC) was synthesized as a coating material in magnetic dispersive solidphase microextraction (M-dSPmE) technique to monitor the copper and lead levels in lake water, seawater, wastewater and vegetable matrices including radish, spinach, lettuce, and celery products.In the mentioned study, the various analytical parameters, including sample pH, eluent, and sample volume, were adjusted to optimize the technique. 150

Advantages and limitations of SPME
SPME has emerged as a powerful tool in modern analytical technology, providing advantages over traditional extraction procedures.Importantly, its extraordinary automation and high-throughput capabilities reduce the general analysis time, making it an affordable option for several laboratories with large volumes of samples.Furthermore, SPME improves the accuracy and precision by reducing the possibility of error through a streamlined workow. 151PME provides various advantages over traditional methods, such as liquid-liquid extraction (LLE) in environmental analysis. 152To begin with, its capacity to adsorb analytes onto the sorbent ber effortlessly allows for safe storage and transport purposes, especially for delicate or volatile compounds.This eliminates concerns about degradation during transit and facilitates eld-based sampling.Moreover, SPME facilitates environmentally responsible operations by decreasing sample volume and eliminating huge solvent volumes, resulting in lower waste disposal costs.In addition, SPME enables tailored extraction by selectively interacting the analyte with the speci-ed sorbent.This technique successfully preconcentrates the low volatile compounds and increases the range of analyzable species and enhances recovery efficiency over LLE. 153Finally, the selective adsorption process leads to signicantly higher enrichment factors compared to LLE, resulting in improved detection limits and sensitivity, particularly for trace analysis. 154espite its numerous advantages, SPME presents a few limitations that need to be carefully considered.For instance, the inter-batch and inter-manufacturer variability in ber necessitates optimization before each analysis to achieve reliable and accurate results.Furthermore, the intrinsic fragility of the SPME bers requires careful handling and conditioning protocols to prevent both physical damage and potential desorption of the sorbent coatings. 155Subsequently, gas bubble formation on the ber surface may affect the mass transfer rates.Employing an internal standards with mass spectrometry increases the accuracy of the data but at an additional cost involved. 156Additionally, the limiting capacity of the ber causes the sensitivity of SPME to peak at a specic sample volume.Selecting sorbent material may lead to matrix interferences or competitive binding among the analytes for limited binding sites that are challenging for accurate quantication, particularly in case of complex matrices.Depending on the analyte properties or complexity of the matrix, the efficiency of analyte desorption from the ber aer adsorption can vary.The sensitivity may be lower compared to some traditional techniques, especially for analytes with low affinity for the sorbent. 157inally, optimizing specic analytes and matrices can be timeconsuming and require expertise and it may hinder widespread adoption. 158By carefully addressing its limits and maximizing its benets, SPME has enormous abilities to revolutionize analytical workows and improve the accuracy, efficiency, and cost-effectiveness of modern research and analysis.

Conclusion
In conclusion, solid phase microextraction has emerged as a promising eco-friendly sample preparation method for environmental analysis.Its simplicity, accuracy, and integration of sampling and preconcentration in a single step have made it a popular choice among researchers.Moreover, the use of nanomaterials, metal-organic frameworks, and carbon materials as active materials has further widened the research area of this green analytical technique.As compared to traditional techniques like solid phase extraction, SPME offers several advantages for its simplicity, versatility, minimum solvent usage, and reduced sample handing process.However, several areas still require further research, such as the development of suitable methods for complex matrices, new types of coating materials, and the detection of multiple compounds by a single device.The development of an effective coating recipe for SPME will also facilitate minimising the matrix effect while working with soil and sediment samples.The development of a highthroughput SPME method is to be considered for simultaneous extraction and monitoring of pollutants from environmental matrices.Despite its advantages, researchers must also consider the possibility of incomplete extraction and loss of accuracy in complex matrices like soil samples.Therefore, further development, such as incorporating a new functional group in coating materials, is crucial to successfully integrate the SPME technique for environmental analysis.In the future, investigators may consider the current challenges and contribute to designing cost-effective and efficient SPME devices for the determination of environmental pollution levels.

Table 1
Applications of SPME fibers for environmental analysis

Table 2
Various applications of SPME for environmental gas samples analysis © 2024 The Author(s).Published by the Royal Society of Chemistry RSC Adv., 2024, 14, 27608-27621 | 27613

Table 3
Application of SPME for aqueous samples analysis

Table 4
122lications of SPME for soil and sediment samples Scientists developed an advanced special hollow-ber membraneprotected SPME technique for analyzing herbicides in sewage sludge samples.122MorganW. Conrady et al. developed an SPME-based method for simultaneous preconcentration and analysis of geosmin and 2-methylisoborneol (2-MIB) from the soil matrix.The study also demonstrated the effectiveness of PDMS ber for the extraction of 2-MIB.